Restructuring at Waters Group in Hertfordshire has seen it close its Peugeot dealership at Hertford.
The business, which has been in the town for 50 years, closes on December 7.
It's not yet known whether other dealership closures are planned.
Waters also represents Peugeot at Hatfield and Lea Valley, Renault at Hatfield and Letchworth and Mazda at Lea Valley.
Waters is part of MCL Group, the former Mazda and Kia distributor which is part of the Japanese Itochu Corporation which also owns Stapletons Tyre Services (STS).
MCL Group also has three car supermarket sites, branded Autoplanet, in London, Southampton and Maidstone.