Used car supermarket The Car Shop will open a site in Doncaster in mid-November.
The company , which already has sites in Northampton, Cardiff and Swindon, said it aims to sell more than 6,000 used cars and vans a year at the new site, which will open with a stock of up to 1,000 vehicles in and around a 140,000 sq ft warehouse.
One hundred jobs will be created from the move.
The Car Shop joint operations director Jonathan Dunkley said: "These are difficult times for most but, so far, 2009 has been a record year for us due to our proactive approach.
"We looked at various areas but chose Doncaster because of the enormous opportunities it presents in sales and staffing potential. We are really pleased to be branching out into Yorkshire and look forward to bringing our brand and business strategy to the region."