Bond drove the DBS in Casino Royale in 2006 and crashed it in style. A stuntman rolled the DBS seven times to break the Guinness world record for 'most cannon roles in a car'.
It is unclear how many of the £160,000 sports cars Aston will be providing to the Bond production team.
Dr Ulrich Bez, Aston Martin chief executive officer, said: "Aston Martin is proud of our long association with James Bond.
"The DBS has its own unique character which equals that of James Bond. It is explosive power in a black tie. It is the perfect car for James Bond as it delivers the complete driving experience and bridges the gap between our road and track cars. It is equally at home on a twisting mountain circuit as on the open road and we are all very excited at the prospect of seeing it on the big screen again."
Bond 22 (working title) is scheduled for international release in November 2008.
To view a clip of the DBS flipping out on the set of Casino Royale click here.